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neweastbio@126.com
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| 價格 | 1600.00 |
| 品牌 | NewEast Biosciences  |
| 產(chǎn)地 | 中國武漢 |
| 貨號 | 26133 |
| 規(guī)格 | 100μl |
| 說明書 | 說明書下載 |
GRB7 [26133]
[100 µL]
| Catalog Number: 26133 |
| Gene Symbol: GRB7 |
| Description: anti GRB7 Mouse Monoclonal Antibody |
| Background: Grb7 is an adaptor protein that interacts with epidermal growth factor receptor (EGFR) and ephrin receptors. It plays a role in the integrin signaling pathway and cell migration by binding with focal adhesion kinase (FAK). Grb7 gene is commonly co-amplified (present in excess copies) in breast cancers. Grb7 is over-expressed in testicular germ cell tumors, esophageal cancers, and gastric cancers. There are clues that it involved in cell migration and metastatic progression of human esophageal carcinomas. |
| Immunogen: Purified GRB7 protein, human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:100-1:1000 IHC: 1:50-1:200 |
| Concentration: 0.5 mg/ml |
| Host: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes GRB7 of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Western blot: |
 |
Western blot analysis of recombinant GRB7 protein. Purified His-tagged GRB7 protein was blotted with anti GRB7 mouse monoclonal antibody (Cat. #26133). |
| Immunohistochemistry: |
|
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with anti GRB7 monoclonal antibody (Cat. #26133). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody. |
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